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KMID : 0380420150390010015
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Journal of Prventive Veterinary Medicine
2015 Volume.39 No. 1 p.15 ~ p.22
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Effects of calcium on the formation of preneoplastic lesions in a mouse model of colon carcinogenesis
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Myung Yun-Jung
Seol Ja-Kyung
Jeong Jae-Hwang
Nam Sang-Yoon
Yun Young-Won
Kim Jong-Soo
Lee Beom-Jun
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Abstract
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Calcium exerts antiproliferative effects on cellular targets through the promotion of differentiation and apoptosis. Weinvestigated the influence of calcium on the formation of colonic aberrant crypt foci (ACFs), which were induced by exposureto azoxymethane (AOM) followed by dextran sodium sulfate (DSS), in ICR mice. Six-week-old ICR mice received 3 (weeks0?2) intraperitoneal injections of AOM (10 mg/kg BW), followed by treatment with 2% DSS via drinking water for a weekto induce preneoplastic lesions. The mice were then divided into 3 groups: the control (AOM/DSS), AOM/DSS + 1.0% Ca,and AOM/DSS + 2.0% Ca groups. Calcium (1.0 or 2.0%) was administered via drinking water for 12 weeks. After sacrificingthe mice, the total numbers of aberrant crypts (ACs) and ACFs were measured in the colonic mucosa after methylene bluestaining. The control group displayed 11.58 ± 2.43 ACFs/colon, which were composed of a total of 30.42 ± 5.18 ACs/colon. The number of ACFs with more than 3 ACs, which are likely to progress to colon cancer, was 2.37 ± 0.68. Compared tothe control, 1.0% or 2.0% calcium treatment significantly decreased the number of total ACFs and ACs in aconcentration-dependent manner. The decrease in ACFs or ACs after calcium treatment was associated with decreases incell proliferation and β-catenin expression and an increase in apoptosis in colonic mucosal cells. These results suggest thatcalcium may exert a protective effect against colon cancer by inhibiting the development of ACFs/ACs in ICR mice.
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KEYWORD
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calcium, colon cancer, azoxymethane, aberrant crypt foci, apoptosis
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